pL-rpb1-N488D
(Plasmid
#10988)
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 10988 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepRS415 (with modifications)
- Backbone size w/o insert (bp) 4967
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Vector typeBacterial Expression, Yeast Expression
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Selectable markersLEU2
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namerpb1-N488D
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Alt nameRPB1
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SpeciesS. cerevisiae (budding yeast)
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Insert Size (bp)6349
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MutationMutation 1462 A to G in the RPB1 ORF resulting in the residue substitution N488D
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Entrez GeneRPO21 (a.k.a. YDL140C, RPB1, RPB220, SIT1, SUA8)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site XhoI/AvaI (not destroyed)
- 3′ cloning site ApaI (not destroyed)
- 5′ sequencing primer M13 reverse
- 3′ sequencing primer n/a (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pL-rpb1-N488D was a gift from Jeff Strathern (Addgene plasmid # 10988 ; http://n2t.net/addgene:10988 ; RRID:Addgene_10988) -
For your References section:
Mutations in the Saccharomyces cerevisiae RPB1 Gene Conferring Hypersensitivity to 6-Azauracil. Malagon F, Kireeva ML, Shafer BK, Lubkowska L, Kashlev M, Strathern JN. Genetics. 2006 Apr . 172(4):2201-9. 10.1534/genetics.105.052415 PubMed 16510790