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Addgene

pFLAG/HA-TNRC6B
(Plasmid #10979)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 10979 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    N/A
  • Backbone size w/o insert (bp) 5324
  • Vector type
    Mammalian Expression
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    TNRC6B
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    5169
  • GenBank ID
    NM_015088
  • Entrez Gene
    TNRC6B (a.k.a. GDSBA)
  • Tags / Fusion Proteins
    • FLAG (N terminal on insert)
    • HA (N terminal on insert)

Cloning Information

  • Cloning method Gateway Cloning
  • 5′ cloning site attR1 (not destroyed)
  • 3′ cloning site attR2 (not destroyed)
  • 5′ sequencing primer T7
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pFLAG/HA-TNRC6B was a gift from Thomas Tuschl (Addgene plasmid # 10979 ; http://n2t.net/addgene:10979 ; RRID:Addgene_10979)

  • For your References section:

    Identification of Novel Argonaute-Associated Proteins. Meister G, Landthaler M, Peters L, Chen PY, Urlaub H, Luhrmann R, Tuschl T. Curr Biol. 2005 Nov 9. ():. 10.1016/j.cub.2005.10.048 PubMed 16289642
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