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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 10977 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepDEST26
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Backbone manufacturerInvitrogen
- Backbone size w/o insert (bp) 5882
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameMOV10
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SpeciesH. sapiens (human)
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Insert Size (bp)3012
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GenBank IDNP_066014
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Entrez GeneMOV10 (a.k.a. fSAP113, gb110)
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Tag
/ Fusion Protein
- myc (N terminal on insert)
Cloning Information
- Cloning method Gateway Cloning
- 5′ cloning site attR1 (not destroyed)
- 3′ cloning site attR2 (not destroyed)
- 5′ sequencing primer CMV-F (Common Sequencing Primers)
Resource Information
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pmyc-MOV10 was a gift from Thomas Tuschl (Addgene plasmid # 10977 ; http://n2t.net/addgene:10977 ; RRID:Addgene_10977) -
For your References section:
Identification of Novel Argonaute-Associated Proteins. Meister G, Landthaler M, Peters L, Chen PY, Urlaub H, Luhrmann R, Tuschl T. Curr Biol. 2005 Nov 9. ():. 10.1016/j.cub.2005.10.048 PubMed 16289642