pcDNA4 myc PGC-1 alpha
(Plasmid #10974)

• Purpose: Mammalian expression of PGC-1a with myc and his tags
• Depositing Lab: Toren Finkel
• Publication: Ichida et al J Biol Chem. 2002 Dec 27. 277(52):50991-5.

Backbone

• Vector backbone: pcDNA4 myc his
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 5075
• Vector type: Mammalian Expression
• Selectable markers: Zeocin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: PGC-1 alpha
• Alt name: PPARGC1
• Species: H. sapiens (human)
• Insert Size (bp): 2400
• GenBank ID: NP_037393
• Entrez Gene: PPARGC1A (a.k.a. LEM6, PGC-1(alpha), PGC-1alpha, PGC-1v, PGC1, PGC1A, PPARGC1)
• Tags / Fusion Proteins:
  • Myc (C terminal on backbone)
  • His (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ sequencing primer: T7
• 3′ sequencing primer: BGHRev

Resource Information

• Addgene Notes:
  • Addgene Diagnostic Digest
• Articles Citing this Plasmid:
  • 28 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry