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pcDNA3.1his p66shc S36A
(Plasmid #10973)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 10973 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3.1 His
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5500
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    p66shc
  • Species
    H. sapiens (human)
  • Mutation
    S36A
  • Entrez Gene
    SHC1 (a.k.a. SHC, SHCA)
  • Tag / Fusion Protein
    • His (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer BGHrev
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please note that this plasmid contains additional mutations (D2N and S38P) in the p66/SHC sequence when compared to GenBank entries NP_892113.4 and NP_001123512.1. This is the original plasmid as used in the associated publication listed below.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA3.1his p66shc S36A was a gift from Toren Finkel (Addgene plasmid # 10973 ; http://n2t.net/addgene:10973 ; RRID:Addgene_10973)

  • For your References section:

    Redox regulation of forkhead proteins through a p66shc-dependent signaling pathway. Nemoto S, Finkel T. Science. 2002 Mar 29. 295(5564):2450-2. 10.1126/science.1069004 PubMed 11884717
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