pcDNA3.1his p66shc S36A
(Plasmid #10973)

• Depositing Lab: Toren Finkel
• Publication: Nemoto et al Science. 2002 Mar 29. 295(5564):2450-2.

Backbone

• Vector backbone: pcDNA3.1 His
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 5500
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: p66shc
• Species: H. sapiens (human)
• Mutation: S36A
• Entrez Gene: SHC1 (a.k.a. SHC, SHCA)
• Tag / Fusion Protein:
  • His (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (not destroyed)
• 3′ cloning site: EcoRI (not destroyed)
• 5′ sequencing primer: T7
• 3′ sequencing primer: BGHrev

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please note that this plasmid contains additional mutations (D2N and S38P) in the p66/SHC sequence when compared to GenBank entries NP_892113.4 and NP_001123512.1. This is the original plasmid as used in the associated publication listed below.