pTA-Luc SIRT1 promoter (-202)
(Plasmid #10971)

• Depositing Lab: Toren Finkel
• Publication: Nemoto et al Science. 2004 Dec 17. 306(5704):2105-8.

Backbone

• Vector backbone: pTA-Luc
• Backbone manufacturer: Clontech
• Backbone size w/o insert (bp): 4800
• Vector type: Mammalian Expression, Luciferase

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: SIRT1 promoter
• Species: M. musculus (mouse)
• Insert Size (bp): 202
• Entrez Gene: Sirt1 (a.k.a. SIR2L1, Sir2, Sir2a, Sir2alpha)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ sequencing primer: RVprimer3
• 3′ sequencing primer: LucNrev

Resource Information

• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Luciferase Limited Use Label License
• Industry Terms:
  • Not Available to Industry