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Addgene

pcDNA3.1hisA cdc25c C377S
(Plasmid #10967)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 10967 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pcDNA3.1 His A
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5500
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    cdc25c C377S
  • Alt name
    cdc25c
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1450
  • Mutation
    C377S. Cysteine to Serine substitution at aa377.
  • Entrez Gene
    CDC25C (a.k.a. CDC25, PPP1R60)
  • Tag / Fusion Protein
    • 6xHis (N terminal on backbone)

Cloning Information

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    cdc25C was a gift from Dr. Helen Piwnica-Worms

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA3.1hisA cdc25c C377S was a gift from Toren Finkel (Addgene plasmid # 10967)

  • For your References section:

    Redox regulation of Cdc25C. Savitsky PA, Finkel T. J Biol Chem. 2002 Jun 7. 277(23):20535-40. 10.1074/jbc.M201589200 PubMed 11925443
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