pcDNA3.1hisA cdc25c C330S C377S
(Plasmid #10965)

• Depositing Lab: Toren Finkel
• Publication: Savitsky et al J Biol Chem. 2002 Jun 7. 277(23):20535-40.

Backbone

• Vector backbone: pcDNA3.1 His A
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 5500
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: cdc25c C330S C377S
• Alt name: cdc25c
• Species: H. sapiens (human)
• Insert Size (bp): 1450
• Mutation: C330S C377S. Cysteine to Serine substitution at aa330 and aa377.
• Entrez Gene: CDC25C (a.k.a. CDC25, PPP1R60)
• Tag / Fusion Protein:
  • 6xHis (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ sequencing primer: T7
• 3′ sequencing primer: BGHrev

Resource Information

• A portion of this plasmid was derived from a plasmid made by: cdc25C was a gift from Dr. Helen Piwnica-Worms

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry