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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 10963 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepCruz HA
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Backbone manufacturerSanta Cruz Biotechnology
- Backbone size w/o insert (bp) 6600
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameSIRT1
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SpeciesM. musculus (mouse)
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MutationG261A
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Entrez GeneSirt1 (a.k.a. SIR2L1, Sir2, Sir2a, Sir2alpha)
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Tag
/ Fusion Protein
- HA (N terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer T7 (Common Sequencing Primers)
Resource Information
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCruzHA SIRT1 G261A was a gift from Toren Finkel (Addgene plasmid # 10963 ; http://n2t.net/addgene:10963 ; RRID:Addgene_10963) -
For your References section:
SIRT1 functionally interacts with the metabolic regulator and transcriptional coactivator PGC-1{alpha}. Nemoto S, Fergusson MM, Finkel T. J Biol Chem. 2005 Apr 22. 280(16):16456-60. 10.1074/jbc.M501485200 PubMed 15716268