pCruzHA SIRT1
(Plasmid #10962)

• Depositing Lab: Toren Finkel
• Publication: Nemoto et al J Biol Chem. 2005 Apr 22. 280(16):16456-60.

Backbone

• Vector backbone: pCruz HA
• Backbone manufacturer: Santa Cruz Biotechnology
• Backbone size w/o insert (bp): 6600
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: SIRT1
• Species: M. musculus (mouse)
• Entrez Gene: Sirt1 (a.k.a. SIR2L1, Sir2, Sir2a, Sir2alpha)
• Tag / Fusion Protein:
  • HA (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ sequencing primer: CMV-F
• 3′ sequencing primer: BGH-rev

Resource Information

• Articles Citing this Plasmid:
  • 9 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please note that Addgene's sequencing results identified a G->S and A->G mutations in the SIRT1 sequence when compared to GenBank entries NP_062786.1, NP_001153062.1 and NP_001153061.1 (which correspond to isoforms 1-3, respectively). This plasmid is the original sample that was used in the associated publication listed below.