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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 10958 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepcDNA3.1 mychis A
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Backbone manufacturerInvitrogen
- Backbone size w/o insert (bp) 5500
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namexanthine dehydrogenase
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Alt namexdh
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SpeciesH. sapiens (human)
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Insert Size (bp)4000
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GenBank IDNP_035853
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Entrez GeneXDH (a.k.a. XAN1, XO, XOR)
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Tags
/ Fusion Proteins
- his (C terminal on backbone)
- myc (C terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer T7
- 3′ sequencing primer BGHrev (Common Sequencing Primers)
Resource Information
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pcDNA3.1mychisA XDH was a gift from Toren Finkel (Addgene plasmid # 10958 ; http://n2t.net/addgene:10958 ; RRID:Addgene_10958) -
For your References section:
Xanthine oxidoreductase is an endogenous regulator of cyclooxygenase-2. Ohtsubo T, Rovira II, Starost MF, Liu C, Finkel T. Circ Res. 2004 Nov 26. 95(11):1118-24. 10.1161/01.RES.0000149571.96304.36 PubMed 15528468