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Addgene

pcDNA3.1 NM23-H1
(Plasmid #10956)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 10956 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5400
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    NM23
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    500
  • Entrez Gene
    NME1 (a.k.a. AWD, GAAD, NB, NBS, NDKA, NDPK-A, NDPKA, NM23, NM23-H1)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer T7
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA3.1 NM23-H1 was a gift from Patricia Steeg (Addgene plasmid # 10956 ; http://n2t.net/addgene:10956 ; RRID:Addgene_10956)

  • For your References section:

    Evidence for a novel gene associated with low tumor metastatic potential. Steeg PS, Bevilacqua G, Kopper L, Thorgeirsson UP, Talmadge JE, Liotta LA, Sobel ME. J Natl Cancer Inst. 1988 Apr 6. 80(3):200-4. 10.1093/jnci/80.3.200 PubMed 3346912
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