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Addgene

pME-actn2
(Plasmid #109550)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 109550 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pDEST-actc1b-mKate2-ccdb
  • Vector type
    Zebrafish plasmids

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    alpha actinin 2
  • Species
    D. rerio (zebrafish)
  • Mutation
    Please see Depositor Comments

Cloning Information

  • Cloning method Gateway Cloning

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Addgene NGS found R52H, S412T, R500K, S567P, V721A, and E770K amino acid mutations within the alpha actinin 2 translation that should not affect plasmid function

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pME-actn2 was a gift from Rob Parton (Addgene plasmid # 109550 ; http://n2t.net/addgene:109550 ; RRID:Addgene_109550)

  • For your References section:

    In vivo cell biological screening identifies an endocytic capture mechanism for T-tubule formation. Hall TE, Martel N, Ariotti N, Xiong Z, Lo HP, Ferguson C, Rae J, Lim YW, Parton RG. Nat Commun. 2020 Jul 24;11(1):3711. doi: 10.1038/s41467-020-17486-w. 10.1038/s41467-020-17486-w PubMed 32709891
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