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Addgene

pUC8 mouse IFN-gamma
(Plasmid #10955)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 10955 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pUC8
  • Backbone manufacturer
    Sigma
  • Backbone size w/o insert (bp) 2665
  • Vector type
    cloning vector

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    interferon-gamma
  • Alt name
    IFN-gamma genomic DNA
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    10500
  • Entrez Gene
    Ifng (a.k.a. IFN-g, If2f, Ifg)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer M13 Reverse
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Mouse genomic DNA containing the IFN-gamma gene. Young lab stock #D3067.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pUC8 mouse IFN-gamma was a gift from Howard Young (Addgene plasmid # 10955 ; http://n2t.net/addgene:10955 ; RRID:Addgene_10955)