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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 10955 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepUC8
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Backbone manufacturerSigma
- Backbone size w/o insert (bp) 2665
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Vector typecloning vector
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameinterferon-gamma
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Alt nameIFN-gamma genomic DNA
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SpeciesM. musculus (mouse)
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Insert Size (bp)10500
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Entrez GeneIfng (a.k.a. IFN-g, If2f, Ifg)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (not destroyed)
- 3′ cloning site BamHI (not destroyed)
- 5′ sequencing primer M13 Reverse (Common Sequencing Primers)
Resource Information
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Mouse genomic DNA containing the IFN-gamma gene. Young lab stock #D3067.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pUC8 mouse IFN-gamma was a gift from Howard Young (Addgene plasmid # 10955 ; http://n2t.net/addgene:10955 ; RRID:Addgene_10955)