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Addgene

pSV2neo human IFN-gamma
(Plasmid #10954)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 10954 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pSV2neo
  • Backbone manufacturer
    ATCC
  • Backbone size w/o insert (bp) 5500
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    interferon-gamma
  • Alt name
    IFN-gamma
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    8600
  • Entrez Gene
    IFNG (a.k.a. IFG, IFI, IMD69)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer AGT AAC AGC TTC CCA CAT CA
  • 3′ sequencing primer EBV-rev
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Human genomic DNA containing the IFN-gamma gene, approximately 2.7 kb upstream and 1 kb downstream of exons, poly A site. Young lab stock #D3126.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pSV2neo human IFN-gamma was a gift from Howard Young (Addgene plasmid # 10954 ; http://n2t.net/addgene:10954 ; RRID:Addgene_10954)

  • For your References section:

    Expression of transfected human interferon-gamma DNA: evidence for cell-specific regulation. Young HA, Dray JF, Farrar WL. J Immunol. 1986 Jun 15. 136(12):4700-3. PubMed 3011901
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