actc1b-mKate2-ARF6-DN
(Plasmid
#109538)
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PurposeMuscle specific zebrafish expression of mKate2 fused to dominant negative ARF6
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 109538 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepDEST-actc1b-mKate2-ccdb
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Vector typeZebrafish plasmids
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameARF6
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SpeciesD. rerio (zebrafish)
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MutationDN
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Entrez Genearf6a (a.k.a. arf6, wu:fj46d09, zgc:77665)
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Tag
/ Fusion Protein
- mKate2 (N terminal on insert)
Cloning Information
- Cloning method Gateway Cloning
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
actc1b-mKate2-ARF6-DN was a gift from Rob Parton (Addgene plasmid # 109538 ; http://n2t.net/addgene:109538 ; RRID:Addgene_109538) -
For your References section:
In vivo cell biological screening identifies an endocytic capture mechanism for T-tubule formation. Hall TE, Martel N, Ariotti N, Xiong Z, Lo HP, Ferguson C, Rae J, Lim YW, Parton RG. Nat Commun. 2020 Jul 24;11(1):3711. doi: 10.1038/s41467-020-17486-w. 10.1038/s41467-020-17486-w PubMed 32709891
