pEGFP-TRAIL
(Plasmid #10953)

• Depositing Lab: Bingliang Fang
• Publication: Kagawa et al Cancer Res. 2001 Apr 15. 61(8):3330-8.

Backbone

• Vector backbone: pEGFP-C3
• Backbone size w/o insert (bp): 4727
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Tumor necrosis factor alpha related apoptosis inducing ligand
• Alt name: TRAIL
• Species: H. sapiens (human)
• Insert Size (bp): 836
• Mutation: NA
• Entrez Gene: TNFSF10 (a.k.a. APO2L, Apo-2L, CD253, TANCR, TL2, TNLG6A, TRAIL)
• Tag / Fusion Protein:
  • GFP (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (not destroyed)
• 3′ cloning site: SacII (not destroyed)
• 5′ sequencing primer: EGFP-C

Resource Information

• Articles Citing this Plasmid:
  • 14 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry