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Addgene

actc1b-mKate2-EHD1a-dEH
(Plasmid #109513)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 109513 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pDEST-actc1b-mKate2-ccdb
  • Vector type
    Zebrafish plasmids

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    EHD1a
  • Species
    D. rerio (zebrafish)
  • Mutation
    dEH
  • Entrez Gene
    zgc:77049 (a.k.a. MGC77049)
  • Tag / Fusion Protein
    • mKate2 (N terminal on insert)

Cloning Information

  • Cloning method Gateway Cloning

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    actc1b-mKate2-EHD1a-dEH was a gift from Rob Parton (Addgene plasmid # 109513 ; http://n2t.net/addgene:109513 ; RRID:Addgene_109513)

  • For your References section:

    In vivo cell biological screening identifies an endocytic capture mechanism for T-tubule formation. Hall TE, Martel N, Ariotti N, Xiong Z, Lo HP, Ferguson C, Rae J, Lim YW, Parton RG. Nat Commun. 2020 Jul 24;11(1):3711. doi: 10.1038/s41467-020-17486-w. 10.1038/s41467-020-17486-w PubMed 32709891
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