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Addgene

mCarmine in pcDNA3
(Plasmid #109486)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 109486 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5400
  • Total vector size (bp) 6100
  • Modifications to backbone
    recombination sequences added for seamless ligation cloning
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    mCarmine
  • Species
    Synthetic; Entactameaa
  • Insert Size (bp)
    1
  • Promoter CMV

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

mCarmine is a far red emitting fluorescent protein (excitation: 603; emission: 675)

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    mCarmine in pcDNA3 was a gift from Oliver Griesbeck (Addgene plasmid # 109486 ; http://n2t.net/addgene:109486 ; RRID:Addgene_109486)

  • For your References section:

    Imaging-Based Screening Platform Assists Protein Engineering. Fabritius A, Ng D, Kist AM, Erdogan M, Portugues R, Griesbeck O. Cell Chem Biol. 2018 Dec 20;25(12):1554-1561.e8. doi: 10.1016/j.chembiol.2018.08.008. Epub 2018 Sep 13. 10.1016/j.chembiol.2018.08.008 PubMed 30220597
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