pOBCol2.3MCSpA
(Plasmid
#109485)
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Purpose(Empty Backbone) Drive inserted gene in osteoblasts.
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 109485 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepUC18
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Backbone manufacturerUnknown
- Backbone size (bp) 2700
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Modifications to backboneHind3 and EcoR1 sites in MCS were destroyed and converted to Sal1 sites with adapters.
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Vector typeMammalian Expression
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Tag
/ Fusion Protein
- None
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer M13forward
- 3′ sequencing primer M13reverse (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pOBCol2.3MCSpA was a gift from David Rowe (Addgene plasmid # 109485 ; http://n2t.net/addgene:109485 ; RRID:Addgene_109485) -
For your References section:
Use of type I collagen green fluorescent protein transgenes to identify subpopulations of cells at different stages of the osteoblast lineage. Kalajzic I, Kalajzic Z, Kaliterna M, Gronowicz G, Clark SH, Lichtler AC, Rowe D. J Bone Miner Res. 2002 Jan;17(1):15-25. doi: 10.1359/jbmr.2002.17.1.15. 10.1359/jbmr.2002.17.1.15 PubMed 11771662
Map uploaded by the depositor.
