mCarmine in pRSETB
(Plasmid #109484)

• Purpose: bacterial expression vector for mCarmine, a far red emitting fluorescent protein (excitation: 603; emission: 675)
• Depositing Lab: Oliver Griesbeck
• Publication: Fabritius et al Cell Chem Biol. 2018 Dec 20;25(12):1554-1561.e8. doi: 10.1016/j.chembiol.2018.08.008. Epub 2018 Sep 13.

Backbone

• Vector backbone: pRSETB
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 2900
• Total vector size (bp): 3600
• Modifications to backbone: recombination sites inserted for seamless ligation cloning
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Growth instructions: use BL21 for protein production
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: mCarmine
• Species: Synthetic; Entactamaea
• Insert Size (bp): 1
• Promoter: T7
• Tag / Fusion Protein:
  • N-terminal histidine tag in vector

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: T7
• 3′ sequencing primer: pRSET reverse

Resource Information

• Supplemental Documents:
  • mCarmine_pRSETSLIII.GBK

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

mCarmine is a far red emitting fluorescent protein (excitation: 603; emission: 675)