plenti-CMV-mCherry-T2A-GFP
(Plasmid #109427)

• Purpose: A lentiviral backbone expressing mCherry-T2A-eGFP off of a CMV promoter.
• Depositing Lab: Reuben Harris
• Publication: St Martin et al Nucleic Acids Res. 2018 May 9. pii: 4994269. doi: 10.1093/nar/gky332.

Backbone

• Vector backbone: HIV-1 NL4-3
• Backbone size w/o insert (bp): 9268
• Modifications to backbone: Removed gag-pol, vif, vpr, and part of env from HIV-1 NL4-3 backbone. Inserted mCherry T2A GFP.
• Vector type: Lentiviral

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: mCherry T2A GFP
• Species: Synthetic
• Insert Size (bp): 1503
• Promoter: CMV

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (not destroyed)
• 3′ cloning site: KpnI (not destroyed)
• 5′ sequencing primer: CTGGCTACCGGTATGGTGAGCAAGGGCGAGG
• 3′ sequencing primer: CTTGTACTCGAGATCTGCACCGGGCTTGTACAGCTCGTCCATGCC

Resource Information

• Articles Citing this Plasmid:
  • 4 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry