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Addgene

pBbS8k-RFP_R46Q
(Plasmid #109414)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 109414 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pSC101
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH10b
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    repA
  • Species
    Synthetic
  • Mutation
    R46Q repA mutation to increase copy number
  • Tag / Fusion Protein
    • RFP (N terminal on backbone)

Cloning Information

  • Cloning method Gibson Cloning

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBbS8k-RFP_R46Q was a gift from Jay Keasling (Addgene plasmid # 109414 ; http://n2t.net/addgene:109414 ; RRID:Addgene_109414)

  • For your References section:

    Isolation and characterization of novel mutations in the pSC101 origin that increase copy number. Thompson MG, Sedaghatian N, Barajas JF, Wehrs M, Bailey CB, Kaplan N, Hillson NJ, Mukhopadhyay A, Keasling JD. Sci Rep. 2018 Jan 25;8(1):1590. doi: 10.1038/s41598-018-20016-w. 10.1038/s41598-018-20016-w PubMed 29371642
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