Biomolecular FeedBack (FB)
(Plasmid
#109391)
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PurposeThis construct encodes for two units. The first contains a constitutively expressed dCas9 protein. The second contains an sgRNA cassette under the control of the burden-responsive htpG1 promoter.
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 109391 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepAZ16 ( Plasmid has got AmpR and p15A origin)
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert namepJ23100mut-dCas9-T- phtpG1-sgRNA
Cloning Information
- Cloning method Ligation Independent Cloning
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
Biomolecular FeedBack (FB) was a gift from Tom Ellis (Addgene plasmid # 109391 ; http://n2t.net/addgene:109391 ; RRID:Addgene_109391) -
For your References section:
Burden-driven feedback control of gene expression. Ceroni F, Boo A, Furini S, Gorochowski TE, Borkowski O, Ladak YN, Awan AR, Gilbert C, Stan GB, Ellis T. Nat Methods. 2018 Mar 26. pii: nmeth.4635. doi: 10.1038/nmeth.4635. 10.1038/nmeth.4635 PubMed 29578536
