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Addgene

pSUM36-MCS2-myc
(Plasmid #109379)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 109379 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pSUM36
  • Backbone manufacturer
    from PMID 9218713
  • Backbone size w/o insert (bp) 5200
  • Total vector size (bp) 5371
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    MCS2-Myc
  • Promoter Kanamycin
  • Tag / Fusion Protein
    • Myc (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site unknown (unknown if destroyed)
  • 3′ cloning site unknown (unknown if destroyed)
  • 5′ sequencing primer M13-fwd
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pSUM36-MCS2-myc was a gift from Nicolai van Oers (Addgene plasmid # 109379 ; http://n2t.net/addgene:109379 ; RRID:Addgene_109379)

  • For your References section:

    Mycobacterial shuttle vectors designed for high-level protein expression in infected macrophages. Eitson JL, Medeiros JJ, Hoover AR, Srivastava S, Roybal KT, Ainsa JA, Hansen EJ, Gumbo T, van Oers NS. Appl Environ Microbiol. 2012 Oct;78(19):6829-37. doi: 10.1128/AEM.01674-12. Epub 2012 Jul 20. 10.1128/AEM.01674-12 PubMed 22820329
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