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Purpose(Empty Backbone) This plasmid expresses dsRed2 and puromycin resistance
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Depositing Labs
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Publication
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 109377 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepLenti-CMV-MCs-SV-puro
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Backbone manufacturerPaul Odgren (Addgene plasmid # 73582)
- Backbone size (bp) 8411
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Modifications to backbonedsRed inserted, from EF.CMV.dsRED2 ( Linzhao Cheng, Addgene plasmid #17619)
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Vector typeMammalian Expression, Lentiviral
- Promoter CMV
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Selectable markersPuromycin
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Tag
/ Fusion Protein
- dsRed2 (C terminal on backbone)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberUnknown
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer CMV-F (Common Sequencing Primers)
Resource Information
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Addgene Notes
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
Plenti-CMV-MCS-RFP-SV-puro was a gift from Jonathan Garlick & Behzad Gerami-Naini (Addgene plasmid # 109377 ; http://n2t.net/addgene:109377 ; RRID:Addgene_109377)
