pEM705 Gsi (Ser)
(Plasmid
#109352)
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PurposeGsX chimera with Gαi C-terminus (Pertussis toxin insensitive mutant)
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 109352 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepEM705
- Backbone size w/o insert (bp) 6002
- Total vector size (bp) 7184
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Vector typeMammalian Expression
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Selectable markersPuromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameGsi chimera
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SpeciesH. sapiens (human)
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Insert Size (bp)1182
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MutationChanged Cys at position 391 to Serine (Pertussis toxin insensitive)
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GenBank ID
- Promoter CAG
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Tag
/ Fusion Protein
- None
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer cttctggcgtgtgaccggcggctc
- 3′ sequencing primer TTGGCAGAGGGAAAAAGATCTCAGTGG (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pEM705 Gsi (Ser) was a gift from Robert Lucas (Addgene plasmid # 109352 ; http://n2t.net/addgene:109352 ; RRID:Addgene_109352) -
For your References section:
A live cell assay of GPCR coupling allows identification of optogenetic tools for controlling Go and Gi signaling. Ballister ER, Rodgers J, Martial F, Lucas RJ. BMC Biol. 2018 Jan 16;16(1):10. doi: 10.1186/s12915-017-0475-2. 10.1186/s12915-017-0475-2 [pii] PubMed 29338718
