pEM705 Gsi (Ser)
(Plasmid #109352)

• Purpose: GsX chimera with Gαi C-terminus (Pertussis toxin insensitive mutant)
• Depositing Lab: Robert Lucas
• Publication: Ballister et al BMC Biol. 2018 Jan 16;16(1):10. doi: 10.1186/s12915-017-0475-2.

Backbone

• Vector backbone: pEM705
• Backbone size w/o insert (bp): 6002
• Total vector size (bp): 7184
• Vector type: Mammalian Expression
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Gsi chimera
• Species: H. sapiens (human)
• Insert Size (bp): 1182
• Mutation: Changed Cys at position 391 to Serine (Pertussis toxin insensitive)
• GenBank ID:
• Promoter: CAG
• Tag / Fusion Protein:
  • None

Cloning Information

• Cloning method: Gibson Cloning
• 5′ sequencing primer: cttctggcgtgtgaccggcggctc
• 3′ sequencing primer: TTGGCAGAGGGAAAAAGATCTCAGTGG

Resource Information

• Supplemental Documents:
  • Gsi Ser.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry