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[pSK497] pLJC5 Flag-Depdc5(AB)
(Plasmid #109341)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 109341 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pLJC5
  • Vector type
    Lentiviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Depdc5
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    5000
  • Mutation
    DIYGD(185-189)GSGSG;EQPLH(371-375)GSGSG
  • Entrez Gene
    DEPDC5 (a.k.a. DEP.5, FFEVF, FFEVF1)
  • Tag / Fusion Protein
    • FLAG (N terminal on insert)

Cloning Information

  • Cloning method Unknown

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    [pSK497] pLJC5 Flag-Depdc5(AB) was a gift from David Sabatini & Kuang Shen (Addgene plasmid # 109341 ; http://n2t.net/addgene:109341 ; RRID:Addgene_109341)

  • For your References section:

    Architecture of the human GATOR1 and GATOR1-Rag GTPases complexes. Shen K, Huang RK, Brignole EJ, Condon KJ, Valenstein ML, Chantranupong L, Bomaliyamu A, Choe A, Hong C, Yu Z, Sabatini DM. Nature. 2018 Apr 5;556(7699):64-69. doi: 10.1038/nature26158. Epub 2018 Mar 28. 10.1038/nature26158 PubMed 29590090
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