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Addgene

pECFP18aaEYFP
(Plasmid #109330)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 109330 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pECFP-C1
  • Backbone manufacturer
    Clontech
  • Backbone size w/o insert (bp) 4731
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    ECFP-EYFP FRET positive control
  • Species
    Synthetic
  • Insert Size (bp)
    773
  • Mutation
    ECFP linked to EYFP with 18 aa linker GLRSRAQASNSAVEGSAM
  • Promoter CMV

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site unknown (unknown if destroyed)
  • 3′ cloning site unknown (unknown if destroyed)
  • 5′ sequencing primer CMV_F
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pECFP18aaEYFP was a gift from Gabriele Kaminski Schierle (Addgene plasmid # 109330 ; http://n2t.net/addgene:109330 ; RRID:Addgene_109330)

  • For your References section:

    A quantitative protocol for intensity-based live cell FRET imaging. Kaminski CF, Rees EJ, Schierle GS. Methods Mol Biol. 2014;1076:445-54. doi: 10.1007/978-1-62703-649-8_19. 10.1007/978-1-62703-649-8_19 PubMed 24108638
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