pYPQ210
(Plasmid
#109329)
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Purpose(Empty Backbone) Multisite Gateway T-DNA entry plasmid (attR1 & attR2); Zea mays Ubi1 promoter and bar gene for plant selection
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 109329 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepMDC123
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Vector typePlant Expression ; Gateway compatible attR1-attR2 destination vector
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Selectable markersBasta
Growth in Bacteria
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Bacterial Resistance(s)Chloramphenicol and Kanamycin, 25 & 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)ccdB Survival
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameNone
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pYPQ210 was a gift from Yiping Qi (Addgene plasmid # 109329 ; http://n2t.net/addgene:109329 ; RRID:Addgene_109329) -
For your References section:
Activities and specificities of CRISPR/Cas9 and Cas12a nucleases for targeted mutagenesis in maize. Lee K, Zhang Y, Kleinstiver BP, Guo JA, Aryee MJ, Miller J, Malzahn A, Zarecor S, Lawrence-Dill CJ, Joung JK, Qi Y, Wang K. Plant Biotechnol J. 2018 Jul 4. doi: 10.1111/pbi.12982. 10.1111/pbi.12982 PubMed 29972722