pYPQ166
(Plasmid #109328)

• Purpose: Gateway entry plasmid (attL1 & attR5) expressing 3_FLAG-NLS-zCas9-NLS without promoter
• Depositing Lab: Yiping Qi
• Publication: Lee et al Plant Biotechnol J. 2018 Jul 4. doi: 10.1111/pbi.12982.

Backbone

• Vector backbone: pYPQ165
• Vector type: Plant Expression, CRISPR ; Gateway compatible zCas9 entry clone

Growth in Bacteria

• Bacterial Resistance(s): Spectinomycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: zCas9
• Alt name: 3xFLAG-NLS-zCas9-NLS
• Mutation: Zea mays codon-optimized Cas9
• Tags / Fusion Proteins:
  • 3x FLAG, NLS (N terminal on insert)
  • NLS (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: unknown (unknown if destroyed)
• 3′ cloning site: unknown (unknown if destroyed)

Resource Information

• Supplemental Documents:
  • pYPQ166.gb
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

3_FLAG-NLS-zCas9-NLS cassette was originally from pHEE401E (Addgene plasmid#71287)