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Addgene

pYPQ165
(Plasmid #109327)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 109327 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pYPQ167
  • Vector type
    Plant Expression, CRISPR ; Gateway compatible zCas9 entry clone

Growth in Bacteria

  • Bacterial Resistance(s)
    Spectinomycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    zCas9
  • Alt name
    3xFLAG-NLS-zCas9-NLS
  • Mutation
    Zea mays codon-optimized Cas9
  • Promoter Promoter EC1.2 enhancer fused to EC1.1 promoter
  • Tags / Fusion Proteins
    • 3x FLAG, NLS (N terminal on insert)
    • NLS (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site unknown (unknown if destroyed)
  • 3′ cloning site unknown (unknown if destroyed)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

pEGG-3_FLAG-NLS-zCas9-NLS cassette was originally from pHEE401E (Addgene plasmid#71287)

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pYPQ165 was a gift from Yiping Qi (Addgene plasmid # 109327 ; http://n2t.net/addgene:109327 ; RRID:Addgene_109327)

  • For your References section:

    Activities and specificities of CRISPR/Cas9 and Cas12a nucleases for targeted mutagenesis in maize. Lee K, Zhang Y, Kleinstiver BP, Guo JA, Aryee MJ, Miller J, Malzahn A, Zarecor S, Lawrence-Dill CJ, Joung JK, Qi Y, Wang K. Plant Biotechnol J. 2018 Jul 4. doi: 10.1111/pbi.12982. 10.1111/pbi.12982 PubMed 29972722
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