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Addgene

hSyn1-AsCpf1(RR)
(Plasmid #109325)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 109325 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    Custom
  • Vector type
    AAV

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    hAsCpf1(RR)
  • Alt name
    Human codon-optimized AsCpf1 (RR variant)
  • Species
    H. sapiens (human)
  • Mutation
    S542R/K607R
  • Promoter hSyn1
  • Tags / Fusion Proteins
    • HA (N terminal on insert)
    • NLS (N terminal on insert)

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    hSyn1-AsCpf1(RR) was a gift from Feng Zhang (Addgene plasmid # 109325 ; http://n2t.net/addgene:109325 ; RRID:Addgene_109325)

  • For your References section:

    Effects of 3D culturing conditions on the transcriptomic profile of stem-cell-derived neurons. Tekin H, Simmons S, Cummings B, Gao L, Adiconis X, Hession CC, Ghoshal A, Dionne D, Choudhury SR, Yesilyurt V, Sanjana NE, Shi X, Lu C, Heidenreich M, Pan JQ, Levin JZ, Zhang F. Nat Biomed Eng. 2018 Jul;2(7):540-554. doi: 10.1038/s41551-018-0219-9. Epub 2018 Apr 9. 10.1038/s41551-018-0219-9 PubMed 30271673
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