pDJSVT87
(Plasmid
#109302)
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Purpose(Empty Backbone) Express periplasmic, outer membrane, or secreted proteins in E. coli - OmpA signal and His6 tag
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 109302 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepACYCDuet-1
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Backbone manufacturerEMD Millipore
- Backbone size (bp) 4008
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Modifications to backboneRemoved DNA between NcoI and XhoI sites and replaced with a new sequence.
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Vector typeBacterial Expression
- Promoter T7
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Tag
/ Fusion Protein
- OmpA 1-27, 6His (N terminal on backbone)
Growth in Bacteria
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Bacterial Resistance(s)Chloramphenicol, 25 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer ACYCDuetUp1
- 3′ sequencing primer T7 terminator (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pDJSVT87 was a gift from Daniel Slade (Addgene plasmid # 109302 ; http://n2t.net/addgene:109302 ; RRID:Addgene_109302) -
For your References section:
A Vector Suite for the Overexpression and Purification of Tagged Outer Membrane, Periplasmic, and Secreted Proteins in E. coli. Casasanta MA, Slade DJ. Methods Mol Biol. 2019;1960:123-138. doi: 10.1007/978-1-4939-9167-9_11. 10.1007/978-1-4939-9167-9_11 PubMed 30798527
Map uploaded by the depositor.
