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pCR2.1-promoter-mIL-6
(Plasmid #109296)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 109296 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCR2.1
  • Backbone manufacturer
    Clontech
  • Backbone size w/o insert (bp) 4700
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    murine IL-6 promoter
  • Alt name
    16193
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    600
  • Mutation
    CMV promoter in pEGFP-N1 replaced with murine IL-6 promoter
  • Entrez Gene
    Il6 (a.k.a. Il-6)
  • Promoter murine IL-6 promoter

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site AseI (unknown if destroyed)
  • 3′ cloning site AgeI (unknown if destroyed)
  • 5′ sequencing primer NA
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCR2.1-promoter-mIL-6 was a gift from Nicolai van Oers (Addgene plasmid # 109296 ; http://n2t.net/addgene:109296 ; RRID:Addgene_109296)

  • For your References section:

    Mycobacterial shuttle vectors designed for high-level protein expression in infected macrophages. Eitson JL, Medeiros JJ, Hoover AR, Srivastava S, Roybal KT, Ainsa JA, Hansen EJ, Gumbo T, van Oers NS. Appl Environ Microbiol. 2012 Oct;78(19):6829-37. doi: 10.1128/AEM.01674-12. Epub 2012 Jul 20. 10.1128/AEM.01674-12 PubMed 22820329
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