Skip to main content
Addgene

pSUM-GroEL-gfp
(Plasmid #109286)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 109286 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pSUM36
  • Backbone manufacturer
    from PMID 9218713
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    GroEL-GFP
  • Mutation
    GFP modified for expression in prokaryotes; includes an optimized translational initiation region (from pGreenTIR)
  • Promoter GroEL

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (unknown if destroyed)
  • 3′ cloning site XbaI (unknown if destroyed)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The groEL promoter was PCR-amplified from M. tuberculosis genomic DNA, cloned into the pCR2.1 TOPO-TA cloning vector and subsequently isolated as a BamHI/XbaI piece that was subcloned into pSUM36 cut with BamHI and NheI to remove the lacZ promoter. The GFP gene was subcloned into the HindIII site of the pSUM-groEL vector.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pSUM-GroEL-gfp was a gift from Nicolai van Oers (Addgene plasmid # 109286 ; http://n2t.net/addgene:109286 ; RRID:Addgene_109286)

  • For your References section:

    Mycobacterial shuttle vectors designed for high-level protein expression in infected macrophages. Eitson JL, Medeiros JJ, Hoover AR, Srivastava S, Roybal KT, Ainsa JA, Hansen EJ, Gumbo T, van Oers NS. Appl Environ Microbiol. 2012 Oct;78(19):6829-37. doi: 10.1128/AEM.01674-12. Epub 2012 Jul 20. 10.1128/AEM.01674-12 PubMed 22820329
Related items:
Commonly requested with: