pKM492-Myc-His
(Plasmid #109283)

• Purpose: ORBIT integrating plasmid for C-terminal tagging with Myc-His tag
• Depositing Lab: Kenan Murphy
• Publication: Murphy et al MBio. 2018 Dec 11;9(6). pii: mBio.01467-18. doi: 10.1128/mBio.01467-18.

Backbone

• Vector backbone: pKM480
• Backbone manufacturer: Kenan Murphy
• Total vector size (bp): 3572
• Modifications to backbone: Insertion of attB-4xGly-TEV-Myc-6xHis
• Vector type: ORBIT integrating plasmid
• Selectable markers: Hygromycin

Growth in Bacteria

• Bacterial Resistance(s): Chloramphenicol, 25 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Growth instructions: Grow in LB containing 20 ug/ml chloramphenicol.
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: attB-Myc-His
• Species: Synthetic
• Insert Size (bp): 121

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Christina Baer's vector pEN-Myc-23 was the source of the insertion.

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

ORBIT plasmid for creating Myc-His fusions to target genes.

Strain should be grown in 20 ug/ml chloramphenicol for isolation of plasmid. Yields with Qiagen miniprep kits are typically in the range from 60-90 ug/ml from 5 ml culture.