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pET28a-RupA_R-NHis
(Plasmid #109257)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 109257 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET28a
  • Backbone manufacturer
    Novagen
  • Backbone size w/o insert (bp) 5369
  • Total vector size (bp) 6523
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    RupA_R
  • Species
    Ruminococcus bromii
  • Insert Size (bp)
    1218
  • Mutation
    contains aa 2038..2443 of WP_015523502.1
  • GenBank ID
    NC_021013.1 WP_015523502.1
  • Promoter T7
  • Tag / Fusion Protein
    • His tag (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NheI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer TAATACGACTCACTATAGGG
  • 3′ sequencing primer GCTAGTTATTGCTCAGCGG
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET28a-RupA_R-NHis was a gift from Emily Balskus (Addgene plasmid # 109257 ; http://n2t.net/addgene:109257 ; RRID:Addgene_109257)

  • For your References section:

    Discovery of small molecule protease inhibitors by investigating a widespread human gut bacterial biosynthetic pathway,. Schneider BA, EP Balskus. Tetrahedron 10.1016/j.tet.2018.03.043
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