pTHSSe_46
(Plasmid
#109244)
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PurposepSC101 var4 BbsI cloning vector
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 109244 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backboneunknown
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namesfGFP
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SpeciesSynthetic
- Promoter PJ23105
Cloning Information
- Cloning method Gibson Cloning
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pTHSSe_46 was a gift from Christopher Voigt (Addgene plasmid # 109244 ; http://n2t.net/addgene:109244 ; RRID:Addgene_109244) -
For your References section:
Engineered promoters enable constant gene expression at any copy number in bacteria. Segall-Shapiro TH, Sontag ED, Voigt CA. Nat Biotechnol. 2018 Mar 19. pii: nbt.4111. doi: 10.1038/nbt.4111. 10.1038/nbt.4111 PubMed 29553576