pTHSSe_39
(Plasmid
#109238)
-
PurposePTac driving TALEsp1
-
Depositing Lab
-
Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 109238 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
-
Vector backboneunknown
-
Vector typeBacterial Expression
Growth in Bacteria
-
Bacterial Resistance(s)Spectinomycin, 50 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberLow Copy
Gene/Insert
-
Gene/Insert nameTALEsp1
-
SpeciesSynthetic
- Promoter PTac
Cloning Information
- Cloning method Gibson Cloning
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pTHSSe_39 was a gift from Christopher Voigt (Addgene plasmid # 109238 ; http://n2t.net/addgene:109238 ; RRID:Addgene_109238) -
For your References section:
Engineered promoters enable constant gene expression at any copy number in bacteria. Segall-Shapiro TH, Sontag ED, Voigt CA. Nat Biotechnol. 2018 Mar 19. pii: nbt.4111. doi: 10.1038/nbt.4111. 10.1038/nbt.4111 PubMed 29553576