pT2K-p53DN-T2A-copGFP
(Plasmid #109229)

• Purpose: For Tol2 transposon-mediated stable expression of dominant negative p53 and copGFP
• Depositing Lab: Martine Roussel
• Publication: Kawauchi et al Oncogene. 2017 Sep 14;36(37):5231-5242. doi: 10.1038/onc.2017.110. Epub 2017 May 15.

Backbone

• Vector backbone: pT2K-CAGGS
• Backbone size w/o insert (bp): 8083
• Vector type: Mammalian Expression
• Selectable markers: copGFP

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: dominant negative p53
• Species: M. musculus (mouse)
• Mutation: dominant negative p53
• Entrez Gene: Trp53 (a.k.a. Tp53, bbl, bfy, bhy, p44, p53)
• Promoter: CAGGS
• Tag / Fusion Protein:
  • copGFP (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: unknown (unknown if destroyed)
• 3′ cloning site: unknown (unknown if destroyed)
• 5′ sequencing primer: unknown

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Evrogen Limited Use Label License for FPs
  • National Institute of Genetics Limited Use Label License for Tol2 System
• Industry Terms:
  • Not Available to Industry