MoClo adapted pUC57-Amp Level 1 position 1
(Plasmid
#109220)
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Purpose(Empty Backbone) Non binary level 1 position 1 vector adapted for MoClo modular cloning useful for particle bombardment transformation of plants
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 109220 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepUC57
- Backbone size (bp) 2287
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Modifications to backboneBackbone was ligated with MoClo cloning cassette from vector pICH47732
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Vector typeSynthetic Biology
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Selectable markersLacZ alpha
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Cloning Information
- Cloning method Restriction Enzyme
Resource Information
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Supplemental Documents
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A portion of this plasmid was derived from a plasmid made byVector backbone supplied by GenScript. Cloning cassette digested from plasmid pICH47732 (Addgene plasmid #48000)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
MoClo adapted pUC57-Amp Level 1 position 1 was a gift from Elena Caro Bernat (Addgene plasmid # 109220 ; http://n2t.net/addgene:109220 ; RRID:Addgene_109220)