pTorPE-IP2.0
(Plasmid
#109217)
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PurposeExpress IP2.0 in E. coli
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 109217 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backboneCustomized Vector
- Total vector size (bp) 5544
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameIP2.0 (Inverse Pericam 2.0)
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Insert Size (bp)1572
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Tag
/ Fusion Protein
- His-tag (N terminal on insert)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pTorPE-IP2.0 was a gift from Takeshi Ishihara (Addgene plasmid # 109217 ; http://n2t.net/addgene:109217 ; RRID:Addgene_109217) -
For your References section:
An improved inverse-type Ca2+ indicator can detect putative neuronal inhibition in Caenorhabditis elegans by increasing signal intensity upon Ca2+ decrease. Hara-Kuge S, Nishihara T, Matsuda T, Kitazono T, Teramoto T, Nagai T, Ishihara T. PLoS One. 2018 Apr 25;13(4):e0194707. doi: 10.1371/journal.pone.0194707. eCollection 2018. 10.1371/journal.pone.0194707 PubMed 29694380