-
Depositing Lab
-
Publication
-
Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 10921 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
-
Vector backboneN/A
- Backbone size w/o insert (bp) 5283
-
Vector typeMammalian Expression
-
Selectable markersPuromycin
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberHigh Copy
Gene/Insert
-
Gene/Insert nameDGCR8
-
SpeciesH. sapiens (human)
-
Insert Size (bp)2439
-
GenBank IDNM_022720
-
Entrez GeneDGCR8 (a.k.a. C22orf12, DGCRK6, Gy1, pasha)
-
Tags
/ Fusion Proteins
- FLAG (N terminal on insert)
- HA (N terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer CMV-F (Common Sequencing Primers)
Resource Information
-
Articles Citing this Plasmid
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
( Back to top)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pFLAG/HA-DGCR8 was a gift from Thomas Tuschl (Addgene plasmid # 10921 ; http://n2t.net/addgene:10921 ; RRID:Addgene_10921) -
For your References section:
The human DiGeorge syndrome critical region gene 8 and Its D. melanogaster homolog are required for miRNA biogenesis. Landthaler M, Yalcin A, Tuschl T. Curr Biol. 2004 Dec 14. 14(23):2162-7. 10.1016/j.cub.2004.11.001 PubMed 15589161
