pEXL flag Smad3
(Plasmid #10920)

• Depositing Lab: Bob Weinberg
• Publication: Liu et al Proc Natl Acad Sci U S A. 1997 Sep 30. 94(20):10669-74.

Backbone

• Vector backbone: pEXL (derived from pEGFP-N1)
• Backbone manufacturer: EGFP-N1 is from Clontech
• Backbone size w/o insert (bp): 4750
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Smad3
• Species: H. sapiens (human)
• Entrez Gene: SMAD3 (a.k.a. HSPC193, HsT17436, JV15-2, LDS1C, LDS3, MADH3, hMAD-3, hSMAD3, mad3)
• Tag / Fusion Protein:
  • flag (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (not destroyed)
• 3′ cloning site: NotI (not destroyed)
• 5′ sequencing primer: CMV-Forward

Resource Information

• Articles Citing this Plasmid:
  • 3 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

This is a modified version of pEGFP-N1 where the GFP is replaced with flag-Smad3.