pGS-CD64-2
(Plasmid
#109191)
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PurposeEncodes full-length engineered CD64 with C-terminal Twin-Strep tag to be expressed in a baculovirus/insect cell expression system
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 109191 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepGS_C_Strep, Addgene no. 101652
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Backbone manufacturerbackbone derived from pACEBac1, Geneva Biotech
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Vector typeInsect Expression
Growth in Bacteria
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Bacterial Resistance(s)Gentamicin, 10 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameFull-length engineered CD64, derived from human CD64
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SpeciesSynthetic
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Insert Size (bp)999
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Mutationengineered for efficient expression, see publication
- Promoter Polyhedrin
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Tag
/ Fusion Protein
- Twin-Strep (C terminal on insert)
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer polh
- 3′ sequencing primer SV40_rev (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pGS-CD64-2 was a gift from Monika Golas (Addgene plasmid # 109191 ; http://n2t.net/addgene:109191 ; RRID:Addgene_109191) -
For your References section:
Vectors for Expression of Signal Peptide-Dependent Proteins in Baculovirus/Insect Cell Systems and Their Application to Expression and Purification of the High-Affinity Immunoglobulin Gamma Fc Receptor I in Complex with Its Gamma Chain. Le LTM, Nyengaard JR, Golas MM, Sander B. Mol Biotechnol. 2018 Jan;60(1):31-40. doi: 10.1007/s12033-017-0041-8. 10.1007/s12033-017-0041-8 [pii] PubMed 29143175
