pSV2 neuT
(Plasmid
#10919)
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 10919 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepSV2-dhfr
- Backbone size w/o insert (bp) 5000
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameneuT
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Alt nameneu
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Alt nameneu transforming
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Alt namep185
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SpeciesR. norvegicus (rat)
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Insert Size (bp)5000
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Entrez GeneErbb2 (a.k.a. HER2/neu, neu)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site HindIII (not destroyed)
- 3′ cloning site SalI (not destroyed)
- 5′ sequencing primer na (Common Sequencing Primers)
Resource Information
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
See author's map. Expressed in NIH cells, but pSV2 neuNT expresses better.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pSV2 neuT was a gift from Bob Weinberg (Addgene plasmid # 10919 ; http://n2t.net/addgene:10919 ; RRID:Addgene_10919) -
For your References section:
Multiple independent activations of the neu oncogene by a point mutation altering the transmembrane domain of p185. Bargmann CI, Hung MC, Weinberg RA. Cell. 1986 Jun 6. 45(5):649-57. 10.1016/0092-8674(86)90779-8 PubMed 2871941
Map uploaded by the depositor.
