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Addgene

pSV2 neuNT
(Plasmid #10918)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 10918 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pSV2 neo
  • Backbone size w/o insert (bp) 4400
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    neuNT
  • Alt name
    neu
  • Alt name
    p185
  • Species
    R. norvegicus (rat)
  • Insert Size (bp)
    5000
  • Entrez Gene
    Erbb2 (a.k.a. HER2/neu, neu)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site HindIII (not destroyed)
  • 3′ cloning site SalI (not destroyed)
  • 5′ sequencing primer na
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

See author's map. The neu cDNA was inserted between the HindIII and SmaI sites of pSV2-neo, removing the neo gene. Has normal neu cDNA as in pSV2 neuN with transforming sequence (1899-2387) of pSV2 neuT. Expressed in NIH cells at higher levels than pSV2 neuT.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pSV2 neuNT was a gift from Bob Weinberg (Addgene plasmid # 10918 ; http://n2t.net/addgene:10918 ; RRID:Addgene_10918)

  • For your References section:

    Multiple independent activations of the neu oncogene by a point mutation altering the transmembrane domain of p185. Bargmann CI, Hung MC, Weinberg RA. Cell. 1986 Jun 6. 45(5):649-57. 10.1016/0092-8674(86)90779-8 PubMed 2871941
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