pCMVTnT-Flp-3xFLAG
(Plasmid
#109167)
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PurposeEncodes yeast full-length Flp with C-terminal 3xFLAG tag to be expressed in a mammalian expression system
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 109167 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepCMVTnT
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Backbone manufacturerPromega
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameFlp
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SpeciesS. cerevisiae (budding yeast)
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Insert Size (bp)1344
- Promoter CMV
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Tag
/ Fusion Protein
- 3xFLAG (C terminal on insert)
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer CMV
- 3′ sequencing primer SV40_rev (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCMVTnT-Flp-3xFLAG was a gift from Monika Golas (Addgene plasmid # 109167 ; http://n2t.net/addgene:109167 ; RRID:Addgene_109167) -
For your References section:
Expression of Flp Protein in a Baculovirus/Insect Cell System for Biotechnological Applications. Jensen IS, Inui K, Drakulic S, Jayaprakash S, Sander B, Golas MM. Protein J. 2017 Aug;36(4):332-342. doi: 10.1007/s10930-017-9724-z. 10.1007/s10930-017-9724-z PubMed 28660316
