pACEBac1-CoRESTfl
(Plasmid #109156)

• Purpose: Encodes human full-length CoREST with C-terminal 3xFLAG tag to be expressed in a baculovirus/insect cell expression system
• Depositing Lab: Monika Golas
• Publication: Inui et al Protein Sci. 2017 May;26(5):997-1011. doi: 10.1002/pro.3142. Epub 2017 Mar 12.

Backbone

• Vector backbone: pACEBac1
• Backbone manufacturer: Geneva Biotech
• Vector type: Insect Expression

Growth in Bacteria

• Bacterial Resistance(s): Gentamicin, 10 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: human full-length CoREST, sequence-optimized for insect cells
• Species: H. sapiens (human)
• Insert Size (bp): 1521
• Entrez Gene: RCOR1 (a.k.a. COREST, RCOR)
• Promoter: Polyhedrin
• Tag / Fusion Protein:
  • 3xFLAG (C terminal on insert)

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: polh
• 3′ sequencing primer: SV40_rev

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry